Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Logo Partenaire

Toulouse Plant Microbe Phenotyping Platform

Microbe Phenotyping

omnilog

The Omnilog robot is an automated incubator, containing a camera. It is capable of high throughput colorimetric screening of 50 x 96-well microtitre plates in parallel, equivalent to 4800 simultaneous experiments. Through measuring all the plates every 15 minutes, it can provide kinetic data on metabolic activity of the microorganisms over several days. Combined with the microplates « Biolog » (phenotyping microarrays) it is a powerful tool for the identification of microorganisms and for the characterisation of their metabolic activity in various environmental conditions : different carbon and nitrogen sources, stress resistance (pH, osmolarity, temperature), antibiotic resistance.

Operation

The operation of the platform consists of kinetic measurements of 96-well microtitre plates for all types of microorganisms with the Omnilog robot. Examples of possible analyses:

  • Microorganism identification
  • Metabolic characterisation of microorganisms
  • Antibiotic sensitivity of microorganisms
  • Identification of gene function (knock-out mutants vs wild-type ; comparison of different strains)
  • Optimisation of bio-procedures...

Details on the functioning of the platform

  • A unique incubation temperature is fixed per week, with the maximum duration of an experiment being 6 days.
  • Temperature range of between 20 - 45°C.
  • Day 1 of the analyses: Tuesday afternoon : arrive with inoculated plates before 16h00.
  • Destruction of the microtitre plates: responsibility of the user at the end of the experiment.
  • Data: to be recovered from the PC on a USB key

Biolog plates:

Expertise on the use of the Biolog microtitre plates is not part of the competence of the platform. The platform only provides the means of measurement. Note that the protocol for using this technology can vary with the microorganism. It is recommended to solicit the experts of Biolog (represented by Awel in France).

Clients of TPMP-Omnilog have run experiments with:

  • Ralstonia solanacearum, beta-proteo bacterium, plant pathogen
  • Shinorhizobium melilotii, alpha-proteo bacterium, plant symbiont
  • Cupriavidus taiwanensis, beta-proteo bacterium, plant symbiont
  • Streptomyces spp., Actinobacteria
  • Sclerotinia sclerotiorum, ascomycete, plant pathogen
  • Pseudomonas aeruginosa, gamma-proteo bacterium, human opportunist and pathogen
  • Oomycetes